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	Provedor de dados BJMBR (4) Autor Araujo,E.G. (4) Guilarducci,C.V.V. (1) Pereira,S.P.F. (1) Santos,R.C.C. (1) Sholl-Franco,A. (1) Torres,P.M.M. (1) Mais... Palavra-chave Retina (3) Cytokines (2) Development (2) Amacrine cholinergic cells (1) CAMP (1) Ganglion cell survival (1) Glial cells (1) Growth factor (1) M1 receptor activity (1) Natural cell death (1) Neuroimmunology (1) Neurotrophic factors (1) Retinal ganglion cells (1) Schwann cells (1) Sciatic nerve (1) Veratridine (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (2) Ano 2001 (1) 1998 (1) 1997 (2) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Conditioned medium from activated spleen cells supports the survival of rat retinal cells in vitro BJMBR Sholl-Franco,A.; Araujo,E.G.. Cytokines are a heterogeneous group of molecules that have been associated with several functions in the nervous system, such as survival and differentiation of neuronal and glial cells. In the present study, we demonstrated that conditioned medium from spleen cells activated with concanavalin A increased neuritogenesis and survival of retinal cells, as measured by biochemical and morphological criteria. Our data showed that conditioned medium induced a five-fold increase in the amount of protein after 120 h in vitro. This effect was not inhibited by the blockade of voltage-dependent L-type calcium channels with 5.0 µM nifedipine. However, the use of an intracellular calcium chelator (15.0 µM BAPTA-AM) inhibited this effect. Our results support the idea... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokines; Growth factor; Neuroimmunology; Retina; Development. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100006 Cyclic AMP increases the survival of ganglion cells in mixed retinal cell cultures in the absence of exogenous neurotrophic molecules, an effect that involves cholinergic activity BJMBR Santos,R.C.C.; Araujo,E.G.. Natural cell death is a well-known degenerative phenomenon occurring during development of the nervous system. The role of trophic molecules produced by target and afferent cells as well as by glial cells has been extensively demonstrated. Literature data demonstrate that cAMP can modulate the survival of neuronal cells. Cultures of mixed retinal cells were treated with forskolin (an activator of the enzyme adenylyl cyclase) for 48 h. The results show that 50 µM forskolin induced a two-fold increase in the survival of retinal ganglion cells (RGCs) in the absence of exogenous trophic factors. This effect was dose dependent and abolished by 1 µM H89 (an inhibitor of protein kinase A), 1.25 µM chelerythrine chloride (an inhibitor of protein kinase C), 50 µM... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ganglion cell survival; CAMP; Amacrine cholinergic cells; M1 receptor activity; Glial cells. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200011 Effects of sciatic-conditioned medium on neonatal rat retinal cells in vitro BJMBR Torres,P.M.M.; Guilarducci,C.V.V.; Araujo,E.G.. Schwann cells produce and release trophic factors that induce the regeneration and survival of neurons following lesions in the peripheral nerves. In the present study we examined the in vitro ability of developing rat retinal cells to respond to factors released from fragments of sciatic nerve. Treatment of neonatal rat retinal cells with sciatic-conditioned medium (SCM) for 48 h induced an increase of 92.5 ± 8.8% (N = 7 for each group) in the amount of total protein. SCM increased cell adhesion, neuronal survival and glial cell proliferation as evaluated by morphological criteria. This effect was completely blocked by 2.5 µM chelerythrine chloride, an inhibitor of protein kinase C (PKC). These data indicate that PKC activation is involved in the effect... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytokines; Neurotrophic factors; Retina; Schwann cells; Sciatic nerve. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100012 Veratridine increases the survival of retinal ganglion cells in vitro BJMBR Pereira,S.P.F.; Araujo,E.G.. Neuronal cell death is an important phenomenon involving many biochemical pathways. This degenerative event has been studied to understand how the cells activate the mechanisms that lead to self-destruction. Target cells and afferent cells play a relevant role in the regulation of natural cell death. We studied the effect of veratridine (1.5, 3.0, 4.5 and 6.0 µM) on the survival of neonatal rat retinal ganglion cells in vitro. Veratridine (3.0 µM), a well-known depolarizing agent that opens the Na+ channel, promoted a two-fold increase in the survival of retinal ganglion cells kept in culture for 48 h. This effect was dose-dependent and was blocked by 1.0 µM tetrodotoxin (a classical voltage-dependent Na+ channel blocker) and 30.0 µM flunarizine (a Na+ and... Tipo: Info:eu-repo/semantics/other Palavras-chave: Veratridine; Retinal ganglion cells; Natural cell death; Development; Retina. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200014 Registros recuperados: 4 Primeira ... 1 ... Última

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